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Last updated: 2016/10/23
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WNT3 polyclonal antibody

  • Catalog # : PAB7032
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of WNT3.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human WNT3.
  • Sequence:
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 39.6
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:2000)
    Western blot (1-3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • WNT3 polyclonal antibody (Cat # PAB7032) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • WNT3 polyclonal antibody (Cat # PAB7032, 2 ug/mL) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Immunocytochemistry
  • Immunofluorescence
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 7473
  • Gene Name:
  • WNT3
  • Gene Alias:
  • INT4,MGC131950,MGC138321,MGC138323
  • Gene Description:
  • wingless-type MMTV integration site family, member 3
  • Gene Summary:
  • The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq
  • Other Designations:
  • WNT-3 proto-oncogene protein
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