Product Browser

Last updated: 2016/12/4

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

NONO polyclonal antibody

  • Catalog # : PAB7027
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NONO.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human NONO.
  • Sequence:
  • C-NRAAPGAEFAPNK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 54.2, 54.5
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (0.1-0.3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NONO polyclonal antibody (Cat # PAB7027) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing human NONO and probed with NONO polyclonal antibody (Cat # PAB7027) (mock transfection in first lane), tested by Origene.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • NONO polyclonal antibody (Cat # PAB7027)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4841
  • Gene Name:
  • NONO
  • Gene Alias:
  • NMT55,NRB54,P54,P54NRB
  • Gene Description:
  • non-POU domain containing, octamer-binding
  • Gene Summary:
  • This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq
  • Other Designations:
  • Nuclear RNA-binding protein, 54-kD,OTTHUMP00000023506,OTTHUMP00000023507,OTTHUMP00000023508,non-POU-domain-containing, octamer-binding,non-Pou domain-containing octamer (ATGCAAAT) binding protein
  • RSS
  • YouTube
  • Linkedin
  • Facebook