NONO polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
NONO polyclonal antibody (Cat # PAB7027) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing human NONO and probed with NONO polyclonal antibody (Cat # PAB7027) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NONO polyclonal antibody (Cat # PAB7027)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NONO.
Immunogen
A synthetic peptide corresponding to C-terminus of human NONO.
Sequence
C-NRAAPGAEFAPNK
Host
Goat
Theoretical MW (kDa)
54.2, 54.5
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NONO polyclonal antibody (Cat # PAB7027) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human NONO and probed with NONO polyclonal antibody (Cat # PAB7027) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NONO polyclonal antibody (Cat # PAB7027)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NONO
Entrez GeneID
4841Protein Accession#
NP_0.31389.3Gene Name
NONO
Gene Alias
NMT55, NRB54, P54, P54NRB
Gene Description
non-POU domain containing, octamer-binding
Omim ID
300084Gene Ontology
HyperlinkGene Summary
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq
Other Designations
Nuclear RNA-binding protein, 54-kD|OTTHUMP00000023506|OTTHUMP00000023507|OTTHUMP00000023508|non-POU-domain-containing, octamer-binding|non-Pou domain-containing octamer (ATGCAAAT) binding protein
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Interactome
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Publication Reference
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Furospinosulin-1, marine spongean furanosesterterpene, suppresses the growth of hypoxia-adapted cancer cells by binding to transcriptional regulators p54nrb and LEDGF/p75.
Arai M, Kawachi T, Kotoku N, Nakata C, Kamada H, Tsunoda S, Tsutsumi Y, Endo H, Inoue M, Sato H, Kobayashi M.
ChemBioChem 2016 Jan; 17(2):181.
Application：WB-Ce, Human, DU145 cells.
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NONO and RALY proteins are required for YB-1 oxaliplatin induced resistance in colon adenocarcinoma cell lines.
Tsofack SP, Garand C, Sereduk C, Chow D, Aziz M, Guay D, Yin HH, Lebel M.
Molecular Cancer 2011 Nov; 10:145.
Application：WB, Human, SW480, HT29 cells.
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PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator.
Brown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U.
Science 2005 Apr; 308(5722):693.
Application：WB, Mouse, Rat, ZT6, ZT18, RAT1 cells.
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Furospinosulin-1, marine spongean furanosesterterpene, suppresses the growth of hypoxia-adapted cancer cells by binding to transcriptional regulators p54nrb and LEDGF/p75.
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