CTNNA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CTNNA1 polyclonal antibody (Cat # PAB7024) (1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing human CTNNA1 and probed with CTNNA1 polyclonal antibody (Cat # PAB7024) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CTNNA1 polyclonal antibody (Cat # PAB7024)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CTNNA1.
Immunogen
A synthetic peptide corresponding to internal region of human CTNNA1.
Sequence
C-KREKQDETQTKIK
Host
Goat
Theoretical MW (kDa)
100
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CTNNA1 polyclonal antibody (Cat # PAB7024) (1 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human CTNNA1 and probed with CTNNA1 polyclonal antibody (Cat # PAB7024) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CTNNA1 polyclonal antibody (Cat # PAB7024)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CTNNA1
Entrez GeneID
1495Protein Accession#
NP_001894.2Gene Name
CTNNA1
Gene Alias
CAP102, FLJ36832
Gene Description
catenin (cadherin-associated protein), alpha 1, 102kDa
Omim ID
116805Gene Ontology
HyperlinkGene Summary
alpha 1
Other Designations
alpha-E-catenin|alpha-catenin|alphaE-catenin|cadherin-associated protein,102kDa|catenin (cadherin-associated protein), alpha 1 (102kD)|catenin, alpha 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Gastric cancer: new genetic developments.
Lynch HT, Grady W, Suriano G, Huntsman D.
Journal of Surgical Oncology 2005 Jun; 90(3):114.
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Gastric cancer: new genetic developments.
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