EXOC7 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EXOC7.
Immunogen
A synthetic peptide corresponding to human EXOC7.
Sequence
C-DNIKNDPDKEYN
Host
Goat
Theoretical MW (kDa)
78.1, 74.7
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001013861.1 and NP_0560.34.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.5-1.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EXOC7 polyclonal antibody (Cat # PAB7019) (0.5 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EXOC7 polyclonal antibody (Cat # PAB7019, 0.5 ug/mL) staining of paraffin embedded human pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EXOC7
Entrez GeneID
23265Protein Accession#
NP_001013861.1;NP_0560.34.2Gene Name
EXOC7
Gene Alias
2-5-3p, DKFZp686J04253, EX070, EXO70, EXOC1, Exo70p, FLJ40965, FLJ46415, YJL085W
Gene Description
exocyst complex component 7
Omim ID
608163Gene Ontology
HyperlinkGene Summary
EXOC7 is a component of the exocyst, which is an evolutionarily conserved octameric protein complex essential for exocytosis. The exocyst targets secretory vesicles at specific domains of the plasma membrane for cell surface expansion and protein secretion (Zuo et al., 2006 [PubMed 17086175]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70.
Xu KF, Shen X, Li H, Pacheco-Rodriguez G, Moss J, Vaughan M.
PNAS 2005 Feb; 102(8):2784.
Application:IF, WB-Ce, Human, HepG2 cells.
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Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70.
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