HSD3B1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HSD3B1.
Immunogen
A synthetic peptide corresponding to human HSD3B1.
Sequence
C-DRHKETLKSKTQ
Host
Goat
Theoretical MW (kDa)
42.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.01-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
HSD3B1 polyclonal antibody (Cat # PAB7013) (0.01 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human HSD3B1 and probed with HSD3B1 polyclonal antibody (Cat # PAB7013) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSD3B1
Entrez GeneID
3283Protein Accession#
NP_000853.1Gene Name
HSD3B1
Gene Alias
HSD3B, HSDB3, SDR11E1
Gene Description
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Omim ID
109715Gene Ontology
HyperlinkGene Summary
O
Other Designations
hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1|short chain dehydrogenase/reductase family 11E, member 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility.
Chang BL, Zheng SL, Hawkins GA, Isaacs SD, Wiley KE, Turner A, Carpten JD, Bleecker ER, Walsh PC, Trent JM, Meyers DA, Isaacs WB, Xu J.
Cancer Research 2002 Mar; 62(6):1784.
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Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility.
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