STAM2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
STAM2 polyclonal antibody (Cat # PAB7010) (1 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Flow Cytometry
STAM2 polyclonal antibody (Cat # PAB7010) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STAM2.
Immunogen
A synthetic peptide corresponding to human STAM2.
Sequence
C-QSIDPTDSKPDSQD
Host
Goat
Theoretical MW (kDa)
58.2
Reactivity
Human
Specificity
This antibody is expected to no cross-reaction with STAM1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Flow Cytometry (10 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STAM2 polyclonal antibody (Cat # PAB7010) (1 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
STAM2 polyclonal antibody (Cat # PAB7010) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — STAM2
Entrez GeneID
10254Protein Accession#
NP_005834.3Gene Name
STAM2
Gene Alias
DKFZp564C047, Hbp, STAM2A, STAM2B
Gene Description
signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Omim ID
606244Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq
Other Designations
Hrs-binding protein|STAM-like protein containing SH3 and ITAM domains 2|signal transducing adaptor molecule 2
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Interactome
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Pathway
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Publication Reference
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Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2.
Kobayashi H, Tanaka N, Asao H, Miura S, Kyuuma M, Semura K, Ishii N, Sugamura K.
The Journal of Biological Chemistry 2005 Mar; 280(11):10468.
Application：WB, Mouse, Mouse embryonic fibroblastoid cell lines.
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Hrs, a mammalian master molecule in vesicular transport and protein sorting, suppresses the degradation of ESCRT proteins signal transducing adaptor molecule 1 and 2.
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