PGRMC1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PGRMC1.
Immunogen
A synthetic peptide corresponding to C-terminus of human PGRMC1.
Sequence
C-EPKDESARKND
Host
Goat
Theoretical MW (kDa)
21.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PGRMC1 polyclonal antibody (Cat # PAB7006) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human PGRMC1 and probed with PGRMC1 polyclonal antibody (Cat # PAB7006) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PGRMC1
Entrez GeneID
10857Protein Accession#
NP_006658.1Gene Name
PGRMC1
Gene Alias
HPR6.6, MPR
Gene Description
progesterone receptor membrane component 1
Omim ID
300435Gene Ontology
HyperlinkGene Summary
Progesterone binding protein is a putative steroid membrane receptor. The protein is expressed predominantly in the liver and kidney. [provided by RefSeq
Other Designations
OTTHUMP00000023897|progesterone binding protein
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Interactome
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Disease
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Publication Reference
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Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells.
Hand RA, Craven RJ.
Journal of Cellular Biochemistry 2003 Oct; 90(3):534.
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Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells.
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