SMAD9 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SMAD9 polyclonal antibody (Cat # PAB7003) (0.3 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescen
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMAD9.
Immunogen
A synthetic peptide corresponding to human SMAD9.
Sequence
C-HSEPLMPHNATYPD
Host
Goat
Theoretical MW (kDa)
48.6
Reactivity
Human
Specificity
This antibody is not expected to cross-react with SMAD1 and SMAD5.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SMAD9 polyclonal antibody (Cat # PAB7003) (0.3 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescenEnzyme-linked Immunoabsorbent Assay
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Gene Info — SMAD9
Entrez GeneID
4093Protein Accession#
NP_005896.1Gene Name
SMAD9
Gene Alias
MADH6, MADH9, SMAD8A, SMAD8B
Gene Description
SMAD family member 9
Omim ID
603295Gene Ontology
HyperlinkGene Summary
SMAD family members, such as SMAD9, transduce signals from members of the TGF-beta (see TGFB1; MIM 190180) superfamily, which regulate growth, differentiation, apoptosis, and development (Nishita et al., 1999 [PubMed 10583507]).[supplied by OMIM
Other Designations
MAD (mothers against decapentaplegic, Drosophila) homolog 9|MAD, mothers against decapentaplegic homolog 9|Mothers against decapentaplegic, drosophila, homolog of, 9|OTTHUMP00000178565|SMAD, mothers against DPP homolog 9
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Interactome
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Pathway
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Disease
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Publication Reference
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Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y, Massagué J.
Cell 2003 Jun; 113(6):685.
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Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
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