GALR3 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GALR3.
Immunogen
A synthetic peptide corresponding to C-terminus of human GALR3.
Sequence
C-QGPEPREGPVHGGE
Host
Goat
Theoretical MW (kDa)
39.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GALR3 polyclonal antibody (Cat # PAB6997) (2 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GALR3 polyclonal antibody (Cat # PAB6997) (3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GALR3
Entrez GeneID
8484Protein Accession#
NP_0.03605.1Gene Name
GALR3
Gene Alias
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Gene Description
galanin receptor 3
Omim ID
603692Gene Ontology
HyperlinkGene Summary
The neuropeptide galanin modulates a variety of physiologic processes including cognition/memory, sensory/pain processing, hormone secretion, and feeding behavior. The human galanin receptors are G protein-coupled receptors that functionally couple to their intracellular effector through distinct signaling pathways. GALR3 is found in many tissues and may be expressed as 1.4-, 2.4-, and 5-kb transcripts [provided by RefSeq
Other Designations
OTTHUMP00000028820|galanin receptor, family member 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299.
Swanson CJ, Blackburn TP, Zhang X, Zheng K, Xu ZQ, Hokfelt T, Wolinsky TD, Konkel MJ, Chen H, Zhong H, Walker MW, Craig DA, Gerald CP, Branchek TA.
PNAS 2005 Nov; 102(48):17489.
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Anxiolytic- and antidepressant-like profiles of the galanin-3 receptor (Gal3) antagonists SNAP 37889 and SNAP 398299.
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