CKB polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CKB.
Immunogen
A synthetic peptide corresponding to human CKB.
Sequence
C-KPSDEHKTDLNPDN
Host
Goat
Theoretical MW (kDa)
42.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CKB polyclonal antibody (Cat # PAB6995) (0.1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CKB
Entrez GeneID
1152Protein Accession#
NP_001814.2Gene Name
CKB
Gene Alias
B-CK, CKBB
Gene Description
creatine kinase, brain
Omim ID
123280Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq
Other Designations
brain creatine kinase|creatine kinase B-chain|creatine kinase-B
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Interactome
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Pathway
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Disease
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Publication Reference
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Altered expression and localization of creatine kinase B, heterogeneous nuclear ribonucleoprotein F, and high mobility group box 1 protein in the nuclear matrix associated with colon cancer.
Balasubramani M, Day BW, Schoen RE, Getzenberg RH.
Cancer Research 2006 Jan; 66(2):763.
Application:WB-Ce, WB-Ti, Human, CaCo-2 cells, Human colon, liver tumors.
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Altered expression and localization of creatine kinase B, heterogeneous nuclear ribonucleoprotein F, and high mobility group box 1 protein in the nuclear matrix associated with colon cancer.
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