DDX6 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DDX6.
Immunogen
A synthetic peptide corresponding to human DDX6.
Sequence
C-AEYHSEPVEDEKP
Host
Goat
Theoretical MW (kDa)
53.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DDX6 polyclonal antibody (Cat # PAB6971) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human DDX6 and probed with DDX6 polyclonal antibody (Cat # PAB6971) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DDX6
Entrez GeneID
1656Protein Accession#
NP_004388.1Gene Name
DDX6
Gene Alias
FLJ36338, HLR2, P54, RCK
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Omim ID
600326Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. [provided by RefSeq
Other Designations
DEAD box-6|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
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Interactome
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Pathway
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Publication Reference
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RNA supply drives physiological granule assembly in neurons.
Karl E. Bauer, Niklas Bargenda, Rico Schieweck, Christin Illig, Inmaculada Segura, Max Harner and Michael A. Kiebler.
Nature Communications 2022 May; 13(1):2781.
Application:IF, WB-Ce, Mouse, Rat, mouse hippocampus,rat neuronal cells.
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Cytoplasmic foci are sites of mRNA decay in human cells.
Cougot N, Babajko S, Seraphin B.
The Journal of Cell Biology 2004 Apr; 165(1):31.
Application:IF, Human, HEK 293 cells.
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RNA supply drives physiological granule assembly in neurons.
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