DCP1A polyclonal antibody
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More Files
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of DCP1A.
Immunogen
A synthetic peptide corresponding to human DCP1A.
Sequence
C-QVLTKNKDNHN
Host
Goat
Theoretical MW (kDa)
63.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
DCP1A polyclonal antibody (Cat # PAB6970) (2 ug/mL) staining of A-431 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DCP1A
Entrez GeneID
55802Protein Accession#
NP_060873.3Gene Name
DCP1A
Gene Alias
FLJ21691, HSA275986, Nbla00360, SMAD4IP1, SMIF
Gene Description
DCP1 decapping enzyme homolog A (S. cerevisiae)
Omim ID
607010Gene Ontology
HyperlinkGene Summary
Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. [provided by RefSeq
Other Designations
DCP1 decapping enzyme homolog A|Smad4-interacting transcriptional co-activator|decapping enzyme hDcp1a|putative protein product of Nbla00360|transcription factor SMIF
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Cytoplasmic foci are sites of mRNA decay in human cells.
Cougot N, Babajko S, Seraphin B.
The Journal of Cell Biology 2004 Apr; 165(1):31.
Application:IF, Human, HEK 293 cells.
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Cytoplasmic foci are sites of mRNA decay in human cells.
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