PDE4B polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PDE4B polyclonal antibody (Cat # PAB6965) (0.5 ug/mL) staining of mouse brain Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot (Transfected lysate)
HEK293 overexpressing human PDE4B and probed with PDE4B polyclonal antibody (Cat # PAB6965) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDE4B.
Immunogen
A synthetic peptide corresponding to human PDE4B.
Sequence
C-DIDIATEDKSPVDT
Host
Goat
Theoretical MW (kDa)
83.3, 64.4, 82.1
Reactivity
Mouse
Specificity
This antibody is expected to recognize all reported isoforms (NP_002591.2, NP_0010.32418.1, NP_0010.32416.1 and NP_0010.32417.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PDE4B polyclonal antibody (Cat # PAB6965) (0.5 ug/mL) staining of mouse brain Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescenceWestern Blot (Transfected lysate)
HEK293 overexpressing human PDE4B and probed with PDE4B polyclonal antibody (Cat # PAB6965) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDE4B
Entrez GeneID
5142Protein Accession#
NP_002591.2;NP_0010.32418.1;NP_0010.32416.1;NP_0010.32417.1Gene Name
PDE4B
Gene Alias
DKFZp686F2182, DPDE4, MGC126529, PDE4B5, PDEIVB
Gene Description
phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
Omim ID
600127Gene Ontology
HyperlinkGene Summary
This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
OTTHUMP00000010652|OTTHUMP00000010656|OTTHUMP00000046896|cAMP-specific 3',5'-cyclic phosphodiesterase 4B|cAMP-specific phosphodiesterase-4 B isoform|dunce-like phosphodiesterase E4|phosphodiesterase 4B, cAMP-specific|phosphodiesterase 4B, cAMP-specific (d
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Interactome
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Pathway
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Disease
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Publication Reference
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Genome Wide Mapping Reveals PDE4B as an IL-2 Induced STAT5 Target Gene in Activated Human PBMCs and Lymphoid Cancer Cells.
Nagy ZS, Ross JA, Rodriguez G, Balint BL, Szeles L, Nagy L, Kirken RA.
PLoS One 2013 Feb; 8(2):e57326.
Application：WB, Human, Lymphoid cells.
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DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling.
Millar JK, Pickard BS, Mackie S, James R, Christie S, Buchanan SR, Malloy MP, Chubb JE, Huston E, Baillie GS, Thomson PA, Hill EV, Brandon NJ, Rain JC, Camargo LM, Whiting PJ, Houslay MD, Blackwood DH, Muir WJ, Porteous DJ.
Science 2005 Nov; 310(5751):1187.
Application：IF, WB-Ce, Human, Lymphoblastoid, SH-SY5Y, Hippocampal primary cells.
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Genome Wide Mapping Reveals PDE4B as an IL-2 Induced STAT5 Target Gene in Activated Human PBMCs and Lymphoid Cancer Cells.
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