ARHGEF5 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARHGEF5.
Immunogen
A synthetic peptide corresponding to human ARHGEF5.
Sequence
C-DKEIDQNSQQEE
Host
Goat
Theoretical MW (kDa)
177
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ARHGEF5 polyclonal antibody (Cat # PAB6963) (0.1 ug/mL) staining of HeLa Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARHGEF5
Entrez GeneID
7984Protein Accession#
NP_005426.2Gene Name
ARHGEF5
Gene Alias
DKFZp686N1969, GEF5, P60, TIM, TIM1
Gene Description
Rho guanine nucleotide exchange factor (GEF) 5
Omim ID
600888Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq
Other Designations
guanine nucleotide regulatory protein TIM|rho guanine nucleotide exchange factor 5|transforming immortalized mammary oncogene
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Interactome
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Disease
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Publication Reference
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Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.
Debily MA, Camarca A, Ciullo M, Mayer C, El Marhomy S, Ba I, Jalil A, Anzisi A, Guardiola J, Piatier-Tonneau D.
Human Molecular Genetics 2004 Feb; 13(3):323.
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Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer.
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