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Last updated: 2022/5/22
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HAVCR2 polyclonal antibody 

  • Catalog # : PAB6962
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of HAVCR2.
  • Immunogen:
  • A synthetic peptide corresponding to human HAVCR2.
  • Sequence:
  • C-KWYSHSKEKIQN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 33.4
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:64000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.1-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • HAVCR2 polyclonal antibody (Cat # PAB6962) (0.1 ug/mL) staining of A549 (1) and (0.5 ug/mL) HepG2 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • HAVCR2 polyclonal antibody (Cat # PAB6962) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • HAVCR2 polyclonal antibody (Cat # PAB6962) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • HAVCR2
  • Gene Alias:
  • FLJ14428,KIM-3,TIM3,TIMD3,Tim-3
  • Gene Description:
  • hepatitis A virus cellular receptor 2
  • Gene Summary:
  • CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.[supplied by OMIM
  • Other Designations:
  • T cell immunoglobulin mucin 3,T cell immunoglobulin mucin-3,kidney injury molecule-3
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