Goat polyclonal antibody raised against synthetic peptide of HAVCR2.
Immunogen:
A synthetic peptide corresponding to human HAVCR2.
Sequence:
C-KWYSHSKEKIQN
Host:
Goat
Theoretical MW (kDa):
33.4
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:64000) Flow Cytometry (10 ug/mL) Immunofluorescence (10 ug/mL) Western Blot (0.1-1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
HAVCR2 polyclonal antibody (Cat # PAB6962) (0.1 ug/mL) staining of A549 (1) and (0.5 ug/mL) HepG2 (2) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
HAVCR2 polyclonal antibody (Cat # PAB6962) Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
HAVCR2 polyclonal antibody (Cat # PAB6962) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.[supplied by OMIM
Other Designations:
T cell immunoglobulin mucin 3,T cell immunoglobulin mucin-3,kidney injury molecule-3