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Last updated: 2016/12/4
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VPS25 polyclonal antibody

  • Catalog # : PAB6937
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of VPS25.
  • Immunogen:
  • A synthetic peptide corresponding to human VPS25.
  • Sequence:
  • C-QPNVDTRQKQ
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 20.7
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (0.5-1.5 ug/mL)
    Immunohistochemistry (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • VPS25 polyclonal antibody (Cat # PAB6937) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • VPS25 polyclonal antibody (Cat # PAB6937) (10 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • VPS25
  • Gene Alias:
  • DERP9,EAP20,FAP20,MGC10540
  • Gene Description:
  • vacuolar protein sorting 25 homolog (S. cerevisiae)
  • Gene Summary:
  • VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
  • Other Designations:
  • vacuolar protein sorting 25
  • Gene Pathway
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