VPS25 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
VPS25 polyclonal antibody (Cat # PAB6937) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS25 polyclonal antibody (Cat # PAB6937) (10 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS25.
Immunogen
A synthetic peptide corresponding to human VPS25.
Sequence
C-QPNVDTRQKQ
Host
Goat
Theoretical MW (kDa)
20.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Western Blot (0.5-1.5 ug/mL)
Immunohistochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VPS25 polyclonal antibody (Cat # PAB6937) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS25 polyclonal antibody (Cat # PAB6937) (10 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS25
Entrez GeneID
84313Protein Accession#
NP_115729.1Gene Name
VPS25
Gene Alias
DERP9, EAP20, FAP20, MGC10540
Gene Description
vacuolar protein sorting 25 homolog (S. cerevisiae)
Omim ID
610907Gene Ontology
HyperlinkGene Summary
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM
Other Designations
vacuolar protein sorting 25
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Interactome
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Pathway
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Disease
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Publication Reference
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The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking.
Vaccari T, Bilder D.
Developmental Cell 2005 Nov; 9(5):687.
Application：IS, Drosophila, Eye.
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The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking.
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