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Last updated: 2022/5/22
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GAPDH polyclonal antibody 

  • Catalog # : PAB6936
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GAPDH.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human GAPDH.
  • Sequence:
  • C-GVNHEKYDNSLK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 36.1
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Immunofluorescence (5-10 ug/mL)
    Immunohistochemistry(2 ug/mL)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • GAPDH polyclonal antibody (Cat # PAB6936) (0.1 ug/mL) staining of Human Liver (1), (0.03 ug/mL) Testes (2), Tonsil (3), (0.1 ug/mL) Mouse Liver (4), (0.03 ug/mL) Rat Heart (5) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • GAPDH polyclonal antibody (Cat # PAB6936) (0.03 ug/mL) staining of HeLa (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminesce
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GAPDH polyclonal antibody (Cat # PAB6936) (2 ug/mL) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Immunofluorescence
  • Immunofluorescence
  • GAPDH polyclonal antibody (Cat # PAB6936) Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2597
  • Gene Name:
  • GAPDH
  • Gene Alias:
  • G3PD,GAPD,MGC88685
  • Gene Description:
  • glyceraldehyde-3-phosphate dehydrogenase
  • Gene Summary:
  • The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174431,OTTHUMP00000174432,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
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