SFN polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SFN polyclonal antibody (Cat # PAB6932) (0.1 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SFN polyclonal antibody (Cat # PAB6932) (2.5 ug/mL) staining of paraffin embedded human skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SFN.
Immunogen
A synthetic peptide corresponding to human SFN.
Sequence
C-DLHTLSEDSYKDST
Host
Goat
Theoretical MW (kDa)
27.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SFN polyclonal antibody (Cat # PAB6932) (0.1 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SFN polyclonal antibody (Cat # PAB6932) (2.5 ug/mL) staining of paraffin embedded human skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SFN
Entrez GeneID
2810Protein Accession#
NP_006133.1Gene Name
SFN
Gene Alias
YWHAS
Gene Description
stratifin
Omim ID
601290Gene Ontology
HyperlinkOther Designations
14-3-3 sigma|OTTHUMP00000004242
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Interactome
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Pathway
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Publication Reference
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Differential gene expression profiling of primary cutaneous melanoma and sentinel lymph node metastases.
Koh SS, Wei JP, Li X, Huang RR, Doan NB, Scolyer RA, Cochran AJ, Binder SW.
Modern Pathology 2012 Jun; 25(6):828.
Application：IHC-P, Human, Melanoma, Lymph node.
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The role of epigenetic inactivation of 14-3-3sigma in human cancer.
Lodygin D, Hermeking H.
Cell Research 2005 Apr; 15(4):237.
Application：IHC, WB, Human, Human cancers, Mammalian cells.
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Differential gene expression profiling of primary cutaneous melanoma and sentinel lymph node metastases.
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