TPH2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TPH2.
Immunogen
A synthetic peptide corresponding to human TPH2.
Sequence
C-NKPNSGKNDDKGNK
Host
Goat
Theoretical MW (kDa)
56.1
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Immunohistochemistry (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TPH2 polyclonal antibody (Cat # PAB6927) (2 ug/mL) staining of paraffin embedded Human Brain Stem. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Immunofluorescence
TPH2 polyclonal antibody (Cat # PAB6927) Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
TPH2 polyclonal antibody (Cat # PAB6927) Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
TPH2 polyclonal antibody (Cat # PAB6927) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — TPH2
Entrez GeneID
121278Protein Accession#
NP_775489.2Gene Name
TPH2
Gene Alias
FLJ37295, MGC138871, MGC138872, NTPH
Gene Description
tryptophan hydroxylase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq
Other Designations
neuronal tryptophan hydroxylase|tryptophan 5-monooxygenase 2
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Pathway
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Disease
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Publication Reference
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Haplotype-based linkage of tryptophan hydroxylase 2 to suicide attempt, major depression, and cerebrospinal fluid 5-hydroxyindoleacetic acid in 4 populations.
Zhou Z, Roy A, Lipsky R, Kuchipudi K, Zhu G, Taubman J, Enoch MA, Virkkunen M, Goldman D.
Archives of General Psychiatry 2005 Oct; 62(10):1109.
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Haplotype-based linkage of tryptophan hydroxylase 2 to suicide attempt, major depression, and cerebrospinal fluid 5-hydroxyindoleacetic acid in 4 populations.
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