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Last updated: 2022/5/22
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TPH2 polyclonal antibody 

  • Catalog # : PAB6927
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of TPH2.
  • Immunogen:
  • A synthetic peptide corresponding to human TPH2.
  • Sequence:
  • C-NKPNSGKNDDKGNK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 56.1
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • TPH2 polyclonal antibody (Cat # PAB6927) (2 ug/mL) staining of paraffin embedded Human Brain Stem. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • TPH2 polyclonal antibody (Cat # PAB6927) Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Immunofluorescence
  • Immunofluorescence
  • TPH2 polyclonal antibody (Cat # PAB6927) Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • TPH2 polyclonal antibody (Cat # PAB6927) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • TPH2
  • Gene Alias:
  • FLJ37295,MGC138871,MGC138872,NTPH
  • Gene Description:
  • tryptophan hydroxylase 2
  • Gene Summary:
  • This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq
  • Other Designations:
  • neuronal tryptophan hydroxylase,tryptophan 5-monooxygenase 2
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