PDE4D polyclonal antibody (Cat # PAB6924) staining (1 ug/mL) of COS cell lysates (25 ug protein) : transfected with human PDE4D1 (1), transfected with human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing human PDE4D2 and probed with PDE4D polyclonal antibody (Cat # PAB6924) (mock transfection in first lane), tested by Origene.
This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins