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Last updated: 2022/5/22
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KPNB1 polyclonal antibody 

  • Catalog # : PAB6909
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of KPNB1.
  • Immunogen:
  • A synthetic peptide corresponding to human KPNB1.
  • Sequence:
  • C-RRSKTNKAKTLAT
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 97.2
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Flow Cytometry (10 ug/mL)
    Immunohistochemistry (5 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • KPNB1 polyclonal antibody (Cat # PAB6909) (0.03 ug/mL) staining of A431 (1), HEK293 (2), Jurkat (3), (0.01 ug/mL) Daudi (4), HeLa (5) and (0.1 ug/mL) Kelly (6) and KNRK (7) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human spleen with KPNB1 polyclonal antibody (Cat # PAB6909)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of U-2 OS cell line with KPNB1 polyclonal antibody (Cat # PAB6909). Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of A431 cells (blue line) with KPNB1 polyclonal antibody (Cat # PAB6909). Permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3837
  • Gene Name:
  • KPNB1
  • Gene Alias:
  • IMB1,IPO1,IPOB,Impnb,MGC2155,MGC2156,MGC2157,NTF97
  • Gene Description:
  • karyopherin (importin) beta 1
  • Gene Summary:
  • Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq
  • Other Designations:
  • importin 1,importin 90,importin beta-1 subunit,karyopherin beta 1,nuclear factor p97
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