Goat polyclonal antibody raised against synthetic peptide of KPNB1.
Immunogen:
A synthetic peptide corresponding to human KPNB1.
Sequence:
C-RRSKTNKAKTLAT
Host:
Goat
Theoretical MW (kDa):
97.2
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:32000) Flow Cytometry (10 ug/mL) Immunohistochemistry (5 ug/mL) Immunofluorescence (10 ug/mL) Western Blot (0.03-0.1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
KPNB1 polyclonal antibody (Cat # PAB6909) (0.03 ug/mL) staining of A431 (1), HEK293 (2), Jurkat (3), (0.01 ug/mL) Daudi (4), HeLa (5) and (0.1 ug/mL) Kelly (6) and KNRK (7) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq