MDM2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
MDM2 polyclonal antibody (Cat # PAB6902) (0.3 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing MDM2 and probed with MDM2 polyclonal antibody (Cat # PAB6902) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MDM2.
Immunogen
A synthetic peptide corresponding to human MDM2.
Sequence
C-DELSGERQRKRHKSD
Host
Goat
Theoretical MW (kDa)
56
Reactivity
Human
Specificity
This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MDM2 polyclonal antibody (Cat # PAB6902) (0.3 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing MDM2 and probed with MDM2 polyclonal antibody (Cat # PAB6902) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MDM2
Entrez GeneID
4193Protein Accession#
NP_002383.2Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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Interactome
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Pathway
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Disease
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Publication Reference
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A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans.
Bond GL, Hu W, Bond EE, Robins H, Lutzker SG, Arva NC, Bargonetti J, Bartel F, Taubert H, Wuerl P, Onel K, Yip L, Hwang SJ, Strong LC, Lozano G, Levine AJ.
Cell 2004 Nov; 119(5):591.
Application：WB, Human, A875, CCF-STTG1, H460, Saos2, T47D, Tera 2 cells.
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A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans.
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