PDK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PDK1 polyclonal antibody (Cat # PAB6898) (1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDK1.
Immunogen
A synthetic peptide corresponding to human PDK1.
Sequence
C-DFKDKSAEDAK
Host
Goat
Theoretical MW (kDa)
49.2
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PDK1 polyclonal antibody (Cat # PAB6898) (1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDK1
Entrez GeneID
5163Protein Accession#
NP_002601.1Gene Name
PDK1
Gene Alias
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Gene Description
pyruvate dehydrogenase kinase, isozyme 1
Omim ID
602524Gene Ontology
HyperlinkGene Summary
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
Other Designations
mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex.
Sarbassov DD, Guertin DA, Ali SM, Sabatini DM.
Science 2005 Feb; 307(5712):1098.
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Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex.
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