SIRT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SIRT3 polyclonal antibody (Cat # PAB6891) (0.2 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing SIRT3 and probed with SIRT3 polyclonal antibody (Cat # PAB6891) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIRT3.
Immunogen
A synthetic peptide corresponding to human SIRT3.
Sequence
C-QRETGKLDGPDK
Host
Goat
Theoretical MW (kDa)
43.6, 28.6
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SIRT3 polyclonal antibody (Cat # PAB6891) (0.2 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing SIRT3 and probed with SIRT3 polyclonal antibody (Cat # PAB6891) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIRT3
Entrez GeneID
23410Protein Accession#
NP_036371.1;NP_001017524.1Gene Name
SIRT3
Gene Alias
SIR2L3
Gene Description
sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Omim ID
604481Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq
Other Designations
mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase|silent mating type information regulation 2, S.cerevisiae, homolog 3|sir2-like 3|sirtuin 3|sirtuin type 3
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Interactome
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Disease
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Publication Reference
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SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes.
Shi T, Wang F, Stieren E, Tong Q.
The Journal of Biological Chemistry 2005 Apr; 280(14):13560.
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SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes.
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