SLC9A3R2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SLC9A3R2 polyclonal antibody (Cat # PAB6889) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SLC9A3R2 polyclonal antibody (Cat # PAB6889, 3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SLC9A3R2.
Immunogen
A synthetic peptide corresponding to human SLC9A3R2.
Sequence
C-DGSAWKQDPFQ
Host
Goat
Theoretical MW (kDa)
37.4
Reactivity
Human
Specificity
This variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SLC9A3R2 polyclonal antibody (Cat # PAB6889) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SLC9A3R2 polyclonal antibody (Cat # PAB6889, 3 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SLC9A3R2
Entrez GeneID
9351Protein Accession#
BAA33216.1Gene Name
SLC9A3R2
Gene Alias
E3KARP, MGC104639, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1
Gene Description
solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
Omim ID
606553Gene Ontology
HyperlinkGene Summary
NHE3 (SLC9A3; MIM 182307) is a sodium/hydrogen exchanger in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 (MIM 604990) and protein kinase A phosphorylation, may play a role in NHE3 regulation.[supplied by OMIM
Other Designations
sodium/hydrogen exchanger|solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2|solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2|solute carrier family 9 isoform 3 regulator 2
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Interactome
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Publication Reference
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cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein.
Cha B, Kim JH, Hut H, Hogema BM, Nadarja J, Zizak M, Cavet M, Lee-Kwon W, Lohmann SM, Smolenski A, Tse CM, Yun C, de Jonge HR, Donowitz M.
The Journal of Biological Chemistry 2005 Apr; 280(17):16642.
Application：IP, WB, Mouse, PS120 fibroblasts.
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cGMP inhibition of Na+/H+ antiporter 3 (NHE3) requires PDZ domain adapter NHERF2, a broad specificity protein kinase G-anchoring protein.
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