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Last updated: 2017/9/24
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CBX3 polyclonal antibody

  • Catalog # : PAB6885
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of CBX3.
  • Immunogen:
  • A synthetic peptide corresponding to human CBX3.
  • Sequence:
  • C-EAFLNSQKAGKEKD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 20.8
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is not expected to cross-react with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western blot (0.3-1.0 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • CBX3 polyclonal antibody (Cat # PAB6885) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • CBX3 polyclonal antibody (Cat # PAB6885) (2.5 ug/mL) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_009207.2;NP_057671.2
  • Gene Name:
  • CBX3
  • Gene Alias:
  • HECH,HP1-GAMMA,HP1Hs-gamma
  • Gene Description:
  • chromobox homolog 3 (HP1 gamma homolog, Drosophila)
  • Gene Summary:
  • At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq
  • Other Designations:
  • HP1 gamma homolog,OTTHUMP00000024529,OTTHUMP00000122519,chromobox homolog 3,heterochromatin protein HP1 gamma,heterochromatin-like protein 1
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