EIF3M polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EIF3M.
Immunogen
A synthetic peptide corresponding to human EIF3M.
Sequence
C-NAWKQNLNKVKN
Host
Goat
Theoretical MW (kDa)
42.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
EIF3M polyclonal antibody (Cat # PAB6882) (0.1 ug/mL) staining of A-549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EIF3M polyclonal antibody (Cat # PAB6882, 5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF3M
Entrez GeneID
10480Protein Accession#
NP_006351.2Gene Name
EIF3M
Gene Alias
B5, FLJ29030, GA17, PCID1, hfl-B5
Gene Description
eukaryotic translation initiation factor 3, subunit M
Omim ID
609641Gene Ontology
HyperlinkGene Summary
HFLB5 encodes a broadly expressed protein containing putative membrane fusion domains that acts as a receptor or coreceptor for entry of herpes simplex virus (HSV) (Perez et al., 2005 [PubMed 15919898]).[supplied by OMIM
Other Designations
B5 receptor|PCI domain containing 1 (herpesvirus entry mediator)|dendritic cell protein|fetal lung protein B5
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Interactome
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Disease
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Publication Reference
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The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection.
Perez-Romero P, Fuller AO.
Journal of Virology 2005 Jun; 79(12):7431.
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The C terminus of the B5 receptor for herpes simplex virus contains a functional region important for infection.
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