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Last updated: 2016/11/27
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PCK2 polyclonal antibody

  • Catalog # : PAB6875
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PCK2.
  • Immunogen:
  • A synthetic peptide corresponding to human PCK2.
  • Sequence:
  • KPWKPGDKEPCAH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 70.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (0.5-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PCK2 polyclonal antibody (Cat # PAB6875) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5106
  • Gene Name:
  • PCK2
  • Gene Alias:
  • PEPCK,PEPCK-M,PEPCK2
  • Gene Description:
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Gene Summary:
  • This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164700,PEP carboxykinase,mitochondrial phosphoenolpyruvate carboxykinase 2,phosphoenolpyruvate carboxylase,phosphopyruvate carboxylase
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