PCK2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
PCK2 polyclonal antibody (Cat # PAB6875) (2 ug/mL) staining of A431 (1), HEK293 (2) (1 ug/mL) HepG2 (3) and (2 ug/mL) NIH3T3 (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
PCK2 polyclonal antibody (Cat # PAB6875) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
PCK2 polyclonal antibody (Cat # PAB6875) Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PCK2.
Immunogen
A synthetic peptide corresponding to human PCK2.
Sequence
KPWKPGDKEPCAH
Host
Goat
Theoretical MW (kDa)
70.7
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
PCK2 polyclonal antibody (Cat # PAB6875) (2 ug/mL) staining of A431 (1), HEK293 (2) (1 ug/mL) HepG2 (3) and (2 ug/mL) NIH3T3 (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
PCK2 polyclonal antibody (Cat # PAB6875) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
PCK2 polyclonal antibody (Cat # PAB6875) Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — PCK2
Entrez GeneID
5106Protein Accession#
NP_004554.2Gene Name
PCK2
Gene Alias
PEPCK, PEPCK-M, PEPCK2
Gene Description
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Omim ID
261650Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000164700|PEP carboxykinase|mitochondrial phosphoenolpyruvate carboxykinase 2|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
Duplus E, Benelli C, Reis AF, Fouque F, Velho G, Forest C.
Biochimie 2003 Dec; 85(12):1257.
-
Expression of phosphoenolpyruvate carboxykinase gene in human adipose tissue: induction by rosiglitazone and genetic analyses of the adipocyte-specific region of the promoter in type 2 diabetes.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com