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Last updated: 2022/5/15
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PCK2 polyclonal antibody 

  • Catalog # : PAB6875
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PCK2.
  • Immunogen:
  • A synthetic peptide corresponding to human PCK2.
  • Sequence:
  • KPWKPGDKEPCAH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 70.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • PCK2 polyclonal antibody (Cat # PAB6875) (2 ug/mL) staining of A431 (1), HEK293 (2) (1 ug/mL) HepG2 (3) and (2 ug/mL) NIH3T3 (4) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • PCK2 polyclonal antibody (Cat # PAB6875) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • PCK2 polyclonal antibody (Cat # PAB6875) Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 5106
  • Gene Name:
  • PCK2
  • Gene Alias:
  • PEPCK,PEPCK-M,PEPCK2
  • Gene Description:
  • phosphoenolpyruvate carboxykinase 2 (mitochondrial)
  • Gene Summary:
  • This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164700,PEP carboxykinase,mitochondrial phosphoenolpyruvate carboxykinase 2,phosphoenolpyruvate carboxylase,phosphopyruvate carboxylase
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