GOLGA3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GOLGA3.
Immunogen
A synthetic peptide corresponding to human GOLGA3.
Sequence
C-HSQSRASKEGPGE
Host
Goat
Theoretical MW (kDa)
167
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GOLGA3 polyclonal antibody (Cat # PAB6866) (0.1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GOLGA3
Entrez GeneID
2802Protein Accession#
NP_005886.2Gene Name
GOLGA3
Gene Alias
GCP170, MEA-2
Gene Description
golgi autoantigen, golgin subfamily a, 3
Omim ID
602581Gene Ontology
HyperlinkGene Summary
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq
Other Designations
Golgi autoantigen, golgin subfamily a, 3|Golgi complex-associated protein of 170 kD|Golgi membrane associated protein|Golgi peripheral membrane protein|SY2/SY10 protein|golgin-160|golgin-165|male enhanced antigen-2
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Interactome
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Disease
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Publication Reference
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The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information.
Hicks SW, Machamer CE.
The Journal of Biological Chemistry 2002 Sep; 277(39):35833.
Application:IF, IP, Human, HeLa cells.
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The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information.
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