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LDB3 polyclonal antibody

  • Catalog # : PAB6864
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of LDB3.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human LDB3.
  • Sequence:
  • C-KSQNKPEDEADE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 77.1
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (0.01-0.03 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • LDB3 polyclonal antibody (Cat # PAB6864) (0.01 ug/mL) staining of human muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • LDB3 polyclonal antibody (Cat # PAB6864) (3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_009009.1;NP_001073583.1;NP_001073584.1;NP_001073585.1
  • Gene Name:
  • LDB3
  • Gene Alias:
  • CYPHER,FLJ35865,KIAA01613,KIAA0613,ORACLE,PDLIM6,ZASP,ldb3z1,ldb3z4
  • Gene Description:
  • LIM domain binding 3
  • Gene Summary:
  • This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 and 2) have C-terminal LIM domains. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020008,OTTHUMP00000020009,PDZ and LIM domain 6
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