TGIF2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TGIF2.
Immunogen
A synthetic peptide corresponding to human TGIF2.
Sequence
C-EMELQKQQDP
Host
Goat
Theoretical MW (kDa)
25.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TGIF2 polyclonal antibody (Cat # PAB6859) (0.3 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TGIF2
Entrez GeneID
60436Protein Accession#
NP_068581.1Gene Name
TGIF2
Gene Alias
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Gene Description
TGFB-induced factor homeobox 2
Omim ID
607294Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA-binding homeobox protein and a transcriptional repressor. The encoded protein appears to repress transcription by recruiting histone deacetylases to TGF beta-responsive genes. This gene is amplified and overexpressed in some ovarian cancers, and mutations in this gene can cause holoprosencephaly. [provided by RefSeq
Other Designations
5'-TG-3' interacting factor 2|OTTHUMP00000030848|OTTHUMP00000030849|TGF(beta)-induced transcription factor 2|TGFB-induced factor 2 (TALE family homeobox)|homeobox protein TGIF2|transcription growth factor-beta-induced factor 2
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Interactome
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Publication Reference
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TGIF2 interacts with histone deacetylase 1 and represses transcription.
Melhuish TA, Gallo CM, Wotton D.
J Biol Chem 2001 Jun; 276(34):32109.
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TGIF2 interacts with histone deacetylase 1 and represses transcription.
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