GDF15 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
GDF15 polyclonal antibody (Cat # PAB6858) (0.1 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Cell lysate)
GDF15 polyclonal antibody (Cat # PAB6858) (0.1g/ml) staining of LNCaP (A) and (0.3 ug/mL) K562 (B) cell lysate (35 ug protein in RIPA buffer).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GDF15 polyclonal antibody (Cat # PAB6858) (7 ug/mL) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry
GDF15 polyclonal antibody (Cat # PAB6858) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), (0.5% Triton). Primary incubation 1 hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GDF15.
Immunogen
A synthetic peptide corresponding to human GDF15.
Sequence
C-QKTDTGVSLQTYDD
Host
Goat
Theoretical MW (kDa)
34.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (7 ug/mL)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
GDF15 polyclonal antibody (Cat # PAB6858) (0.1 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Cell lysate)
GDF15 polyclonal antibody (Cat # PAB6858) (0.1g/ml) staining of LNCaP (A) and (0.3 ug/mL) K562 (B) cell lysate (35 ug protein in RIPA buffer).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GDF15 polyclonal antibody (Cat # PAB6858) (7 ug/mL) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
GDF15 polyclonal antibody (Cat # PAB6858) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), (0.5% Triton). Primary incubation 1 hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — GDF15
Entrez GeneID
9518Protein Accession#
NP_004855.1Gene Name
GDF15
Gene Alias
GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Gene Description
growth differentiation factor 15
Omim ID
605312Gene Ontology
HyperlinkGene Summary
Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
Other Designations
NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|PTGF-beta|prostate differentiation factor
-
Interactome
-
Disease
-
Publication Reference
-
The macrophage inhibitory cytokine integrates AKT/PKB and MAP kinase signaling pathways in breast cancer cells.
Wollmann W, Goodman ML, Bhat-Nakshatri P, Kishimoto H, Goulet RJ Jr, Mehrotra S, Morimiya A, Badve S, Nakshatri H.
Carcinogenesis 2005 May; 26(5):900.
-
The macrophage inhibitory cytokine integrates AKT/PKB and MAP kinase signaling pathways in breast cancer cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com