HRH2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
HRH2 polyclonal antibody (Cat # PAB6856) (1 ug/mL) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
HRH2 polyclonal antibody (Cat # PAB6856) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
HRH2 polyclonal antibody (Cat # PAB6856) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HRH2.
Immunogen
A synthetic peptide corresponding to human HRH2.
Sequence
C-QEEKPLKLQVWSGTE
Host
Goat
Theoretical MW (kDa)
40.1, 44.5
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (10 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
HRH2 polyclonal antibody (Cat # PAB6856) (1 ug/mL) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
HRH2 polyclonal antibody (Cat # PAB6856) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
HRH2 polyclonal antibody (Cat # PAB6856) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — HRH2
Entrez GeneID
3274Protein Accession#
NP_071640.1Gene Name
HRH2
Gene Alias
H2R
Gene Description
histamine receptor H2
Omim ID
142703Gene Ontology
HyperlinkGene Summary
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
gastric receptor 1
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Pathway
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Disease
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Publication Reference
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Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation.
Nakane H, Sonobe Y, Watanabe T, Nakano K.
Inflammation Research 2004 Jul; 53(7):324.
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Histamine: its novel role as an endogenous regulator of Con A-dependent T cell proliferation.
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