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Last updated: 2022/8/14
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HRH2 polyclonal antibody 

  • Catalog # : PAB6856
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of HRH2.
  • Immunogen:
  • A synthetic peptide corresponding to human HRH2.
  • Sequence:
  • C-QEEKPLKLQVWSGTE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 40.1, 44.5
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:128000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • HRH2 polyclonal antibody (Cat # PAB6856) (1 ug/mL) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Immunofluorescence
  • Immunofluorescence
  • HRH2 polyclonal antibody (Cat # PAB6856) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • HRH2 polyclonal antibody (Cat # PAB6856) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 3274
  • Gene Name:
  • HRH2
  • Gene Alias:
  • H2R
  • Gene Description:
  • histamine receptor H2
  • Gene Summary:
  • Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • gastric receptor 1
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