CFL2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CFL2 polyclonal antibody (Cat # PAB6848) (0.003 ug/mL) staining of human muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CFL2.
Immunogen
A synthetic peptide corresponding to human CFL2.
Sequence
C-DIKDRSTLGEK
Host
Goat
Theoretical MW (kDa)
18.7, 18.7
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_068733 and NP_619579).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CFL2 polyclonal antibody (Cat # PAB6848) (0.003 ug/mL) staining of human muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CFL2
Entrez GeneID
1073Protein Accession#
NP_068733.1;NP_619579.1Gene Name
CFL2
Gene Alias
NEM7
Gene Description
cofilin 2 (muscle)
Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
cofilin 2
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Interactome
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Pathway
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Publication Reference
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Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion.
Bracken CP, Li X, Wright JA, Lawrence DM, Pillman KA, Salmanidis M, Anderson MA, Dredge BK, Gregory PA, Tsykin A, Neilsen C, Thomson DW, Bert AG, Leerberg JM, Yap AS, Jensen KB, Khew-Goodall Y, Goodall GJ.
The EMBO Journal 2014 Sep; 33(18):2040.
Application：WB-Ce, Human, MCF-7, MDA-MB-361, MDA-MB-468, T47D, ZR-75-1, HBL-100, HS578T, MDA-MB-231, MDA-MB-435, BT549.
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Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle.
Thirion C, Stucka R, Mendel B, Gruhler A, Jaksch M, Nowak KJ, Binz N, Laing NG, Lochmuller H.
European Journal of Biochemistry 2001 Jun; 268(12):3473.
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Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion.
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