GATA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
GATA1 polyclonal antibody (Cat # PAB6841) (0.3 ug/mL) staining of human PBMC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GATA1.
Immunogen
A synthetic peptide corresponding to human GATA1.
Sequence
C-DAEAYRHSPVFQ
Host
Goat
Theoretical MW (kDa)
42.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GATA1 polyclonal antibody (Cat # PAB6841) (0.3 ug/mL) staining of human PBMC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GATA1
Entrez GeneID
2623Protein Accession#
NP_002040.1Gene Name
GATA1
Gene Alias
ERYF1, GF-1, GF1, NFE1
Gene Description
GATA binding protein 1 (globin transcription factor 1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq
Other Designations
GATA binding protein 1|NF-E1 DNA-binding protein|OTTHUMP00000023259|erythroid transcription factor 1|globin transcription factor 1|transcription factor GATA1
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Interactome
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Disease
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Publication Reference
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Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1.
Li Z, Godinho FJ, Klusmann JH, Garriga-Canut M, Yu C, Orkin SH.
Nature Genetics 2005 Jun; 37(6):613.
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Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1.
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