VPS41 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS41 polyclonal antibody (Cat # PAB6823) (4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS41.
Immunogen
A synthetic peptide corresponding to internal region of human VPS41.
Sequence
C-DHLKKDSQNKTLLK
Host
Goat
Theoretical MW (kDa)
98.5, 95.9
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_055211.1 and NP_542198.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS41 polyclonal antibody (Cat # PAB6823) (4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS41
Entrez GeneID
27072Protein Accession#
NP_055211.1;NP_542198.2Gene Name
VPS41
Gene Alias
HVPS41, HVSP41, hVps41p
Gene Description
vacuolar protein sorting 41 homolog (S. cerevisiae)
Omim ID
605485Gene Ontology
HyperlinkGene Summary
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. [provided by RefSeq
Other Designations
OTTHUMP00000025247|vacuolar assembly protein 41|vacuolar protein sorting 41
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Interactome
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Disease
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Publication Reference
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hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif.
McVey Ward D, Radisky D, Scullion MA, Tuttle MS, Vaughn M, Kaplan J.
Experimental Cell Research 2001 Jul; 267(1):126.
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hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif.
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