VPS11 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS11 polyclonal antibody (Cat # PAB6820) (3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS11.
Immunogen
A synthetic peptide corresponding to internal region of human VPS11.
Sequence
C-HALQEKDTQTKLE
Host
Goat
Theoretical MW (kDa)
108
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
VPS11 polyclonal antibody (Cat # PAB6820) (3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS11
Entrez GeneID
55823Protein Accession#
NP_068375.3Gene Name
VPS11
Gene Alias
END1, PEP5, RNF108, hVPS11
Gene Description
vacuolar protein sorting 11 homolog (S. cerevisiae)
Omim ID
608549Gene Ontology
HyperlinkGene Summary
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq
Other Designations
vacuolar protein sorting 11|vacuolar protein sorting protein 11
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Interactome
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Disease
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Publication Reference
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Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7.
Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C.
The Journal of Biological Chemistry 2001 Aug; 276(31):29393.
Application：IP, IF, WB, Human, Monkey, COS-7, HEK 293 cells.
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Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7.
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