SPP1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SPP1.
Immunogen
A synthetic peptide corresponding to C terminus of human SPP1.
Sequence
C-DPKSKEEDKHLK
Host
Goat
Theoretical MW (kDa)
35.4, 33.8, 32.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SPP1 polyclonal antibody (Cat # PAB6818) staining (0.5 ug/mL) of MOLT 4 cell lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPP1
Entrez GeneID
6696Protein Accession#
NP_000573.1Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions.
Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H.
Cancer Biology & Therapy 2005 Jul; 4(7):740.
Application:IHC-P, WB-Tr, Human, Pancreatic, BxPC-3, Colo-357 cells.
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Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions.
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