NPY5R polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NPY5R.
Immunogen
A synthetic peptide corresponding to internal region of human NPY5R.
Sequence
C-GLSNKENRLEEN
Host
Goat
Theoretical MW (kDa)
50.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NPY5R polyclonal antibody (Cat # PAB6799) (3.8 ug/mL) staining of paraffin embedded human intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Western Blot (Tissue lysate)
NPY5R polyclonal antibody (Cat # PAB6799) (0.3 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NPY5R
Entrez GeneID
4889Protein Accession#
NP_006165.1Gene Name
NPY5R
Gene Alias
NPYR5
Gene Description
neuropeptide Y receptor Y5
Omim ID
602001Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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The gut and energy balance: visceral allies in the obesity wars.
Badman MK, Flier JS.
Science 2005 Mar; 307(5717):1909.
Application:WB, Human, Mouse, Hypothalamus.
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The gut and energy balance: visceral allies in the obesity wars.
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