MIF polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MIF.
Immunogen
A synthetic peptide corresponding to C terminus of human MIF.
Sequence
C-NAANVGWNNSTFA
Host
Goat
Theoretical MW (kDa)
12.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:12800)
Sandwhich ELISA (1.5-3 ug/mL)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MIF polyclonal antibody (Cat # PAB6796) (0.03 ug/mL) staining of human thymus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
MIF polyclonal antibody (Cat # PAB6796) (1.5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL). -
Gene Info — MIF
Entrez GeneID
4282Protein Accession#
NP_002406.1Gene Name
MIF
Gene Alias
GIF, GLIF, MMIF
Gene Description
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
Other Designations
glycosylation-inhibiting factor|phenylpyruvate tautomerase
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Interactome
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Pathway
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Disease
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Publication Reference
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Influence of d-glutamine and d-glutamic acid sequences in optical peptide probes targeted against the cholecystokinin-2/gastrin-receptor on binding affinity, specificity and pharmacokinetic properties.
Kossatz S, Mansi R, Behe M, Czerney P, Hilger I.
EJNMMI Research 2013 Nov; 3(1):75.
Application:IHC-P, Mouse, Epidermoid carcinoma.
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Obesity, macrophage migration inhibitory factor, and weight loss.
Church TS, Willis MS, Priest EL, Lamonte MJ, Earnest CP, Wilkinson WJ, Wilson DA, Giroir BP.
International Journal of Obesity 2005 Jun; 29(6):675.
Application:EIA, Human, Plasma.
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Influence of d-glutamine and d-glutamic acid sequences in optical peptide probes targeted against the cholecystokinin-2/gastrin-receptor on binding affinity, specificity and pharmacokinetic properties.
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