LCN2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LCN2.
Immunogen
A synthetic peptide corresponding to human LCN2.
Sequence
C-AILREDKDPQKMY
Host
Goat
Theoretical MW (kDa)
22.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
LCN2 polyclonal antibody (Cat # PAB6795) (0.03 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
LCN2 polyclonal antibody (Cat # PAB6795, 2.5 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LCN2
Entrez GeneID
3934Protein Accession#
NP_005555.2Gene Name
LCN2
Gene Alias
NGAL
Gene Description
lipocalin 2
Omim ID
600181Gene Ontology
HyperlinkOther Designations
OTTHUMP00000022238|OTTHUMP00000022240|neutrophil gelatinase-associated lipocalin|oncogene 24p3|siderocalin
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Interactome
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Disease
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Publication Reference
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Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery.
Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P.
Lancet 2005 Apr; 365(9466):1231.
Application:ELISA, WB, Human, Urine.
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Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery.
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