FFAR1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
FFAR1 polyclonal antibody (Cat # PAB6794) (0.01 ug/mL) staining of human breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FFAR1.
Immunogen
A synthetic peptide corresponding to human FFAR1.
Sequence
CAARTQGGKSQK
Host
Goat
Theoretical MW (kDa)
31.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FFAR1 polyclonal antibody (Cat # PAB6794) (0.01 ug/mL) staining of human breast lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FFAR1
Entrez GeneID
2864Protein Accession#
NP_005294.1Gene Name
FFAR1
Gene Alias
FFA1R, GPCR40, GPR40
Gene Description
free fatty acid receptor 1
Omim ID
603820Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq
Other Designations
G protein-coupled receptor 40
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Interactome
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Disease
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Publication Reference
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Expression of long-chain fatty acid receptor GPR40 is associated with cancer progression in colorectal cancer: A retrospective study.
Nakashima C, Shingo K, Fujiwara-Tani R, Luo Y, Kawahara I, Goto K, Sasaki T, Fujii K, Ohmori H, Kuniyasu H.
Oncology Letters 2018 Jun; 15(6):8641.
Application：IHC, Human, Human colorectal cancer.
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Tumor necrosis factor α decreases glucagon-like peptide-2 expression by up-regulating G-protein-coupled receptor 120 in Crohn disease.
Tsukahara T, Watanabe K, Watanabe T, Yamagami H, Sogawa M, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T.
The American Journal of Pathology 2015 Jan; 185(1):185.
Application：IHC, WB, Human, Ileal mucosa.
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Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40.
Hardy S, St-Onge GG, Joly E, Langelier Y, Prentki M.
The Journal of Biological Chemistry 2005 Apr; 280(14):13285.
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Expression of long-chain fatty acid receptor GPR40 is associated with cancer progression in colorectal cancer: A retrospective study.
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