MAPK3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
MAPK3 polyclonal antibody (Cat # PAB6789) (0.3 ug/mL) staining of 293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing ERK1 and probed with MAPK3 polyclonal antibody (Cat # PAB6789) (mock transfection in second lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MAPK3.
Immunogen
A synthetic peptide corresponding to human MAPK3.
Sequence
GGEPRRTEGVGP-C
Host
Goat
Theoretical MW (kDa)
43.1, 40.1, 38.3
Reactivity
Human
Specificity
No cross-reactivity expected with ERK2
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MAPK3 polyclonal antibody (Cat # PAB6789) (0.3 ug/mL) staining of 293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing ERK1 and probed with MAPK3 polyclonal antibody (Cat # PAB6789) (mock transfection in second lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPK3
Entrez GeneID
5595Protein Accession#
NP_002737.1Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.
Wang PY, Weng J, Anderson RG.
Science 2005 Mar; 307(5714):1472.
Application：WB, Human, HeLa cells.
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OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.
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