C10orf59 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney lysate (35ug protein in RIPA buffer) with C10orf59 polyclonal antibody (Cat# PAB6786) under 0.1-0.3 ug/mL working concentration.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with C10orf59 polyclonal antibody (Cat# PAB6786) shows cyptoplasmic positivity in epithelial cells of the CT under 2-4 ug/mL working concentration.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of C10orf59.
Immunogen
A synthetic peptide corresponding to amino acids 224-233 of human C10orf59.
Sequence
C-VSIDNKKRNI
Host
Goat
Theoretical MW (kDa)
37.8, 34.9
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_001026879.1and NP_060833.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse kidney lysate (35ug protein in RIPA buffer) with C10orf59 polyclonal antibody (Cat# PAB6786) under 0.1-0.3 ug/mL working concentration.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with C10orf59 polyclonal antibody (Cat# PAB6786) shows cyptoplasmic positivity in epithelial cells of the CT under 2-4 ug/mL working concentration.Enzyme-linked Immunoabsorbent Assay
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Gene Info — C10orf59
Entrez GeneID
55328Protein Accession#
NP_001026879.1;NP_060833.1Gene Name
C10orf59
Gene Alias
FLJ11218, RENALASE
Gene Description
chromosome 10 open reading frame 59
Omim ID
609360Gene Ontology
HyperlinkGene Summary
Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney (Xu et al., 2005 [PubMed 15841207]).[supplied by OMIM
Other Designations
OTTHUMP00000020034|OTTHUMP00000020035|renalase
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Disease
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Publication Reference
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Expression and tissue localization of renalase, a novel soluble FAD-dependent protein, in reproductive/steroidogenic systems.
Zhou M, Liang T, Wang Y, Jin D, Wang J, Jia L, Zhang S.
Molecular Biology Reports 2012 Dec; 40(6):3987.
Application：IHC, Mouse, Mouse steroidogenic, gonads, reproductive tissues.
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Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure.
Xu J, Li G, Wang P, Velazquez H, Yao X, Li Y, Wu Y, Peixoto A, Crowley S, Desir GV.
The Journal of Clinical Investigation 2005 May; 115(5):1275.
Application：IF, IHC, WB, Human, Rat, HEK 293 cells, Human heart, kidney, plasma, Rat tissues, Recombinant protein.
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Expression and tissue localization of renalase, a novel soluble FAD-dependent protein, in reproductive/steroidogenic systems.
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