PDHX polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDHX.
Immunogen
A synthetic peptide corresponding to human PDHX.
Sequence
C-KSFKANLENPIRLA
Host
Goat
Theoretical MW (kDa)
54.2, 51.5, 29.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-1 ug/mL)
Immunohistochemistry (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PDHX polyclonal antibody (Cat # PAB6779) (1 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PDHX polyclonal antibody (Cat # PAB6779) staining of paraffin embedded human pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3 ug/mL), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1 ug/mL), HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDHX
Entrez GeneID
8050Protein Accession#
NP_0.03468.1Gene Name
PDHX
Gene Alias
DLDBP, E3BP, OPDX, PDX1, proX
Gene Description
pyruvate dehydrogenase complex, component X
Gene Ontology
HyperlinkGene Summary
The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
Other Designations
E3-binding protein|pyruvate dehydrogenase complex, lipoyl-containing component X
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Interactome
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Publication Reference
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Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms.
Roche TE, Baker JC, Yan X, Hiromasa Y, Gong X, Peng T, Dong J, Turkan A, Kasten SA.
Progress in Nucleic acid Research and Molecular Biology 2001 Jan; 70:33.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms.
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