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PDHX polyclonal antibody

  • Catalog # : PAB6779
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PDHX.
  • Immunogen:
  • A synthetic peptide corresponding to human PDHX.
  • Sequence:
  • C-KSFKANLENPIRLA
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 54.2, 51.5, 29.9
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.1-1 ug/mL)
    Immunohistochemistry (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PDHX polyclonal antibody (Cat # PAB6779) (1 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • PDHX polyclonal antibody (Cat # PAB6779) staining of paraffin embedded human pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3 ug/mL), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1 ug/mL), HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8050
  • Gene Name:
  • PDHX
  • Gene Alias:
  • DLDBP,E3BP,OPDX,PDX1,proX
  • Gene Description:
  • pyruvate dehydrogenase complex, component X
  • Gene Summary:
  • The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex, see MIM 300502. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 (MIM 238331) component of the PDH complex (Robinson et al., 1990 [PubMed 2112155]; Aral et al., 1997 [PubMed 9399911]).[supplied by OMIM
  • Other Designations:
  • E3-binding protein,pyruvate dehydrogenase complex, lipoyl-containing component X
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