AGRP polyclonal antibody
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Immunohistochemistry (Frozen sections)
AGRP polyclonal antibody (Cat # PAB6775) (0.05 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.
Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AGRP.
Immunogen
A synthetic peptide corresponding to C-terminus of human AGRP.
Sequence
CRKLGTAMNPCSRT
Host
Goat
Theoretical MW (kDa)
14.4, 14.4
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (as represented by NP_001129, NP_015531).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Frozen sections) (0.05-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
AGRP polyclonal antibody (Cat # PAB6775) (0.05 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80°C for 30 min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.
Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AGRP
Entrez GeneID
181Protein Accession#
NP_001129.1;NP_015531.1Gene Name
AGRP
Gene Alias
AGRT, ART, ASIP2, MGC118963
Gene Description
agouti related protein homolog (mouse)
Gene Ontology
HyperlinkGene Summary
Agouti-related protein is an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation. Agouti-related protein is alternatively spliced into 2 variants which differ in 5' untranslated sequence length. [provided by RefSeq
Other Designations
Agouti-related transcript, mouse, homolog of|agouti related protein homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus.
Breen TL, Conwell IM, Wardlaw SL.
Brain Research 2005 Jan; 1032(1-2):141.
Application：RIA, Rat, Hypothalamus culture media.
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Effects of fasting, leptin, and insulin on AGRP and POMC peptide release in the hypothalamus.
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