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Last updated: 2016/12/4
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SERPINE1 polyclonal antibody

  • Catalog # : PAB6774
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SERPINE1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human SERPINE1.
  • Sequence:
  • C-GFKIDDKGMAPALRH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 45.1
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognise both reported isoforms.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Sandwhich ELISA (1.5-3 ug/mL)
    Western Blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • SERPINE1 polyclonal antibody (Cat # PAB6774) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • SERPINE1 polyclonal antibody (Cat # PAB6774) (4 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • ELISA
  • Sandwich ELISA
  • Sandwich ELISA
  • SERPINE1 polyclonal antibody (Cat # PAB6774) (1.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5054
  • Protein Accession#:
  • NP_000593.1;NP_001158885.1
  • Gene Name:
  • SERPINE1
  • Gene Alias:
  • PAI,PAI-1,PAI1,PLANH1
  • Gene Description:
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • Gene Summary:
  • This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • plasminogen activator inhibitor, type I,plasminogen activator inhibitor-1,serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
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