OSBP polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of OSBP.
Immunogen
A synthetic peptide corresponding to human OSBP.
Sequence
C-KHQLEETKKEKRTRIP
Host
Goat
Theoretical MW (kDa)
89.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
OSBP polyclonal antibody (Cat # PAB6769) (0.1 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — OSBP
Entrez GeneID
5007Protein Accession#
NP_002547Gene Name
OSBP
Gene Alias
OSBP1
Gene Description
oxysterol binding protein
Omim ID
167040Gene Ontology
HyperlinkGene Summary
Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.
Wang PY, Weng J, Anderson RG.
Science 2005 Mar; 307(5714):1472.
Application:WB, Human, HeLa cells.
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OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.
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