NANOS1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NANOS1.
Immunogen
A synthetic peptide corresponding to human NANOS1.
Sequence
C-SARDGPPGKKLR
Host
Goat
Theoretical MW (kDa)
30.2
Reactivity
Human
Specificity
This antibody is expected to recognize the longer isoform 1 only (NP_955631), ie not isoform 2 (NP_001009553).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NANOS1 polyclonal antibody (Cat # PAB6760) (0.3 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NANOS1
Entrez GeneID
340719Protein Accession#
NP_955631.1Gene Name
NANOS1
Gene Alias
NOS1
Gene Description
nanos homolog 1 (Drosophila)
Omim ID
608226Gene Ontology
HyperlinkOther Designations
OTTHUMP00000046853|nanos homolog 1
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Publication Reference
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Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.
Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA.
Development Genes and Evolution 2003 Apr; 213(3):120.
Application:IHC-P, WB-Ti, Human, Human testis sections.
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Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells.
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