PUM2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human PUM2 with DYKDDDDK tag probed with PUM2 polyclonal antibody (Cat # PAB6759) (0.5 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with PUM2 polyclonal antibody (Cat # PAB6759) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PUM2.
Immunogen
A synthetic peptide corresponding to internal region of human PUM2.
Sequence
C-DQNRDLKQGDDDDSK
Host
Goat
Theoretical MW (kDa)
114
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing human PUM2 with DYKDDDDK tag probed with PUM2 polyclonal antibody (Cat # PAB6759) (0.5 ug/mL) in Lane 1 and probed with anti- DYKDDDDK Tag (1/3000) in lane 3. Mock-transfected HEK293 probed with PUM2 polyclonal antibody (Cat # PAB6759) (1 mg/mL) in Lane 2. Primary incubations were for 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PUM2
Entrez GeneID
23369Protein Accession#
NP_056132.1Gene Name
PUM2
Gene Alias
FLJ36528, KIAA0235, MGC138251, MGC138253, PUMH2, PUML2
Gene Description
pumilio homolog 2 (Drosophila)
Omim ID
607205Gene Ontology
HyperlinkOther Designations
OTTHUMP00000151186|pumilio homolog 2
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Interactome
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Disease
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Publication Reference
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Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation.
Szakmary A, Cox DN, Wang Z, Lin H.
Current Biology 2005 Jan; 15(2):171.
Application：IF, IHC, Drosophila, Drosophila ovarian germline stem cells.
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Regulatory relationship among piwi, pumilio, and bag-of-marbles in Drosophila germline stem cell self-renewal and differentiation.
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